Part:BBa_K1993033

CXCR5-T2A-Luciferase

With the purpose of increasing homing efficiency of MSCs and illustrating their distribution, we had constructed a composite part that containing a chemokine receptor CXCR5 and a bioluminescence Luciferase. It’s also convenient for us to construct our plasmids through Gateway technology.

CXCR5 is responsible for homing to the lesions whose ligand is CXCL13, one of the most common chemokines in various inflammatory diseases, especially in delayed type hypersensitivity(DTH). (Details could be seen on BBa_K1993013).

Luciferase is a kind of oxidative enzymes that produce bioluminescence which enable us to observe cells in vivo. (Details could be seen on BBa_K1993015).

T2A is a self-cleaving 2A peptide (The average length is 18–22 amino acids) that could be applied in expression of more than one gene in cells. (Details could be seen on BBa_K1993019)

Sequence and Features

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BglII site found at 1398
  Illegal BamHI site found at 1941
  Illegal XhoI site found at 31
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 973
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI.rc site found at 259
  Illegal SapI site found at 1050